# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 1 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 1.0 222.3 24.0 0.1 19.2 0.0 1.1 218.5 24.2 0.2 19.2 0.0 1.2 206.6 24.4 0.0 19.3 0.1 1.3 191.2 24.7 0.1 19.7 0.0 1.4 177.3 25.6 0.1 20.6 0.1 1.5 156.4 26.7 0.1 21.6 0.2 1.6 108.3 28.5 0.1 23.4 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 2 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 2.0 201.8 23.7 0.1 18.3 0.1 2.1 186.1 25.5 0.1 20.1 0.0 2.2 150.6 28.4 0.0 23.2 0.1 2.3 95.5 32.9 0.1 27.3 0.1 2.4 46.2 39.3 0.0 33.6 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 3 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 3.0 664.7 24.2 0.1 18.5 0.2 3.1 583.4 25.5 0.1 20.0 0.1 3.2 435.4 29.1 0.1 23.3 0.1 3.3 213.8 36.5 0.1 31.1 0.2 3.4 70.2 44.1 0.3 38.7 0.8 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 4 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 4.0 1002.2 24.0 0.1 18.6 0.2 4.1 959.9 24.6 0.0 19.1 0.1 4.2 681.6 28.5 0.0 23.0 0.1 4.3 413.7 35.0 0.1 29.2 0.2 4.4 95.7 43.7 2.8 39.1 1.0 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 27 01102104 methyl_viologen cell_suspension r._sphaeroides no aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 27.0 349.9 26.0 0.0 20.4 0.1 27.1 304.9 30.0 0.3 22.0 0.0 27.2 282.4 33.3 0.3 24.0 0.1 27.3 253.1 36.3 0.0 25.5 0.0 27.4 231.8 39.3 0.0 27.0 0.1 27.5 216.0 41.5 0.1 28.0 0.0 27.6 199.1 46.4 0.1 30.4 0.0 27.7 189.0 47.7 0.2 31.2 0.0 27.8 185.6 50.4 0.0 32.6 0.1 27.9 167.6 52.8 0.0 33.7 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 28 01102104 methyl_viologen cell_suspension r._sphaeroides no aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 28.0 344.3 25.7 0.0 20.0 0.2 28.1 322.6 29.8 0.2 22.4 0.1 28.2 268.8 32.9 0.0 24.1 0.1 28.3 241.5 35.5 0.2 25.5 0.1 28.4 215.0 38.3 0.1 26.7 0.1 28.5 161.3 42.6 0.1 28.9 0.0 28.6 147.5 45.1 0.0 29.6 0.1